服務(wù)熱線
021-54479081
021-54461587
磷酸化上皮細胞癌轉(zhuǎn)化蛋白2抗體效果
發(fā)布時間：2025/10/27
有效時間：2026/4/26
本產(chǎn)品僅用于科研，不用于臨床
英文名稱 Anti-phospho-ECT2 （Ser370）
中文名稱 磷酸化上皮細胞癌轉(zhuǎn)化蛋白2抗體
別 名 ECT2 （phospho S370）; ECT2 （phospho Ser370）; p-ECT2 （Ser370）; ECT 2; ECT2; ECT-2; ECT2 protein; Epithelial cell transforming sequence 2; Epithelial cell transforming sequence 2 oncogene; Epithelial cell transforming sequence 2 oncogene protein; FLJ10461; MGC138291; Protein ECT2; ECT2_HUMAN; Epithelial cell-transforming sequence 2 oncogene.
濃 度 1mg/1ml
說 明 書 0.1ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human
產(chǎn)品類型 一抗 磷酸化抗體
研究領(lǐng)域
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human ECT2 around the phosphorylation site of Ser370
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. [provided by RefSeq].
Function : Guanine nucleotide exchange factor （GEF） that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits Phenobarbital （PB）-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinay drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
Subunit : Interacts with NR1I3 （By similarity）. Homodimer. Homooligomer. Found in the centralspindlin complex. Interacts （Thr-359 phosphorylated form） with PARD6A; the interaction is observed in cancer cells. Interacts （Thr-359 phosphorylated form） with PRKCI; the interaction is observed in cancer cells. Interacts with PKP4; the interaction is observed at the midbody. Interacts with RACGAP1; the interaction is direct, occurs in a microtubule-dependent manner, is inhibited in metaphase by phosphorylation of ECT2 on Thr-373 and is stimulated in early anaphase by dephosphorylation of ECT2 probably on Thr-373 through CDK1 activity. Interacts with PLK1; the interaction is stimulated upon its phosphorylation on Thr-444. Associates with RACGAP1 at anaphase and during cytokinesis. Interacts with KIF23, PARD3, PARD6A, PARD6B and PRKCQ.
Subcellular Location : Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cleavage furrow. Midbody. Cell junction. Cell junction, tight junction. Note=Sequestered within the nucleus during interphase. Dispersed throughout the cytoplasm upon breakdown of the nuclear envelope during mitosis. Colocalizes with the centralspindlin complex to the mitotic spindles during anaphase/metaphase, the cleavage furrow during ophase and at the midbody at the end of cytokinesis. Colocalized with RhoA at the midbody. Its subcellular localization to tight junction is increased by calcium. Localized predominantly in the cytoplasm of numerous carcinoma cells.
Tissue Specificity : Expressed in lung epithelial cells （at protein level）. Expressed in squamous cell carcinoma, primary non-small cell lung cancer tumors and lung adenocarcinoma. [INDUCTION] Up-regulated by calcium in cells forming cell-cell contact sites. Up-regulated by DNA damaging agents like H（2）O（2） or ionizing radiation （IR）.
Post-translational modifications : Phosphorylated by PLK1 in vitro. Hyperphosphorylated during the G2 phase of the cell cycle. Phosphorylation at Thr-373 occurs during the G2/M phase, relieves its auto-inhibition status and stimulates its GEF activity. Phosphorylation at Thr-444 in G2/M phase is required for subsequent binding with PLK1 and Rho exchange activation. Dephosphorylated at the time of cytokinesis. Phosphorylation at Thr-359 is required for its transformation activity in cancer cells.
Similarity : Contains 2 BRCT domains.
Contains 1 DH （DBL-homology） domain.
Contains 1 PH domain.
Database links : UniProtKB/Swiss-Prot: Q9H8V3.4
宿主：Human、Murine、Rat （Rattus）、Bovine、Dog （Canine）等物種
磷酸化上皮細胞癌轉(zhuǎn)化蛋白2抗體應(yīng)用范圍: WB, ELISA, IP, IHC （p）, IHC （f）, IF
緩沖液：0.01m PBS,pH值7.4和10毫克/毫升BSA
儲存液：0.1%（w/v）
人γ干擾素（IFN-γ）ELISA試劑盒 Human Interferon γ ，IFN-γ ELISA KitY0007496T/48T
人細胞間粘附分子1（ICAM-1/CD54）ELISA試劑盒 Human intercellular adhesion molecule 1，ICAM-1 ELISA KitY0007596T/48T
人肝細胞生長因子（HGF）ELISA試劑盒 Human hepatocyte growth factor，HGF ELISA Kit Y0007696T/48T
人中性粒細胞趨化蛋白2（NAP-2/CXCL7）ELISA試劑盒Human neutrophil activating protein-2，NAP-2 ELISA KitY0007796T/48T
人趨化因子（fractalkine/CX3CL1） ELISA試劑盒Human fractalkine/CX3CL1 ELISA Kit Y0007896T/48T
人堿性成纖維細胞生長因子9（bFGF-9）ELISA試劑盒Human basic fibroblast growth factor 9，bFGF-9 ELISA Kit Y0007996T/48T
人E選擇素（E-Selectin/CD62E）ELISA試劑盒 Human E-Selectin ELISA Kit Y0008096T/48T
人鼠嗜酸粒細胞趨化因子（ECF）ELISA試劑盒Human eosinophil chemotactic factor，ECF ELISA Kit Y0008196T/48T
人CD30分子（CD30）ELISA試劑盒Human Cluster of differentiation 30，CD30 ELISA KitY0008296T/48T
人白細胞活化黏附因子（ALCAM）ELISA試劑盒Human Activated Leukocyte Cell Adhesion Molecule，ALCAM ELISA KitY0008396T/48T
人活化素A（ACV-A）ELISA試劑盒Human Activin A，ACV-A ELISA KitY0008496T/48T
人心鈉肽（ANP）ELISA試劑盒Human Atrial Natriuretic Peptide，ANP ELISA Kit Y0008596T/48T
人內(nèi)嗎啡肽-2（EM-2）ELISA試劑盒Human endomorphin-2，EM-2 ELISA KitY0008696T/48T
人α-內(nèi)嗎啡肽（α-EP）ELISA試劑盒Human α-Endomorphin，α-EP ELISA KitY0008796T/48T
人抑制素（INH）ELISA試劑盒Human Inhibin，INH ELISA Kit Y0008896T/48T
人6-羥多巴胺（6-OHDA）ELISA試劑盒 Human 6-hydroxydopamine，6-OHDA ELISA KitY0008996T/48T
人心納素（ANF）ELISA試劑盒 Human atrial natriuretic factor，ANF ELISA KitY0009096T/48T
人神經(jīng)髓鞘蛋白（p2）ELISA試劑盒 Human myelin protein 2，p2 ELISA KitY0009196T/48T
人神經(jīng)激肽B（NKB）ELISA試劑盒 Human Neurokinins B，NKB ELISA KitY0009296T/48T
人強啡肽（Dyn）ELISA試劑盒Human dynorphin，Dyn ELISA KitY0009396T/48T
人腦啡肽（ENK）ELISA試劑盒Human enkephalin，ENK ELISA KitY0009496T/48T
人神經(jīng)肽Y（NP-Y）ELISA試劑盒Human neuropeptide Y，NP-Y ELISA KitY0009596T/48T
人乙酰膽堿（ACH）ELISA試劑盒Human acetylcholine，ACH ELISA KitY0009696T/48T
人腦鈉素/腦鈉尿肽（BNP）ELISA試劑盒Human brain natriuretic peptide，BNP ELISA KitY0009796T/48T
人細胞角蛋白20（CK20）ELISA試劑盒Human cytokeratin 20，CK20 ELISA KitY0009896T/48T
人N端前腦鈉素（NT-proBNP）ELISA試劑盒Human N-terminal pro-brain natriuretic peptide，NT-proBNP ELISA KitY0009996T/48T
人細胞角蛋白13（CK-13）ELISA試劑盒 Human cytokeratin 13，CK-13 ELISA KitY0010096T/48T
人細胞角蛋白17（CK17）ELISA試劑盒Human cytokeratin 17，CK-17 ELISA Kit Y0010196T/48T
人B細胞淋巴瘤因子3（Bcl3）ELISA試劑盒human B-cell leukemia/lymphoma 3，Bcl3 ELISA KitY0010296T/48T
人P27蛋白（P27）ELISA試劑盒Human P27 protein ELISA KitY0010396T/48T
人粘蛋白/粘液素5B（MUC5B）ELISA試劑盒Human mucin-5 subtype B，MUC5B ELISA KitY0010496T/48T
人Dickkopf 1（DKK1）ELISA試劑盒Human Dickkopf 1，DKK1 ELISA KitY0010596T/48T
人腫瘤標(biāo)志物（CA724）ELISA試劑盒磷酸化上皮細胞癌轉(zhuǎn)化蛋白2抗體 Human CA724 ELISA KitY0010696T/48T
產(chǎn)品中心
Products